Cov khoom
Trimethylol aminomethane
Siv
① Qhov nruab nrab ntawm fosfomycin, kuj tseem siv tau los ua vulcanization accelerator, tshuaj pleev ib ce (cream, lotion), ntxhia roj, paraffin emulsifier, lom tsis haum.
② Siv los ua cov pa roj carbon dioxide, kev npaj ntawm tsis, surfactant, emulsifier thiab accelerator.Nws kuj yog siv rau hauv cov organic synthes.
Txoj kev tsim khoom
Dissolve Tris nrog ib tug me me ntawm ob chav steaming dej (300-500ml), ntxiv HCl, kho pH rau 7.6 nrog HCl (1N) los yog NaOH (1N), thiab thaum kawg ntxiv ob chav steaming dej rau 1000ml.Cov kua no yog cov kua dej cia, khaws cia hauv lub tub yees ntawm 4 ℃.
Nco tseg: Tus nqi PH ntawm Tris-Hcl txawv nrog qhov kub thiab txias, yog li nws yuav tsum ntsuas ntawm chav tsev kub, yog li cov txiaj ntsig ntsuas tau txhim khu kev qha.
Cov yam ntxwv tsis zoo
Tris yog lub hauv paus tsis muaj zog nrog pKa ntawm 8.1 ntawm chav tsev kub (25 ℃).Raws li buffering txoj kev xav, qhov zoo buffering ntau ntawm Tris buffers yog nruab nrab ntawm pH7.0 thiab 9.2.
Lub pH ntawm aqueous tov ntawm Tris puag yog hais txog 10.5, thiab qhov tsis muaj pH tus nqi tuaj yeem tau los ntawm kev ntxiv hydrochloric acid los kho tus nqi pH rau tus nqi xav tau.Txawm li cas los xij, kev saib xyuas yuav tsum tau them rau qhov ntsuas kub ntawm pKa ntawm Tris.Degree, PH tus nqi txo los ntawm 0.03.
1M Tris-HCl 6.8 thiab 1.5M Tris-HCl 8.8 yog cov feem ntau siv reagents rau SDS-PAGE.
Thaum TAE, TBE thiab lwm yam reagents synthesized los ntawm Tris yog cov feem ntau siv reagents rau DNA electrophoresis, TE (pH8.0) yog tsuas yog siv rau DNA dissolution.(TE yog Tris ntxiv rau EDTA.)
Tris buffers tsis yog tsuas yog siv los ua cov kuab tshuaj rau nucleic acids thiab proteins, tab sis kuj muaj ntau yam tseem ceeb siv.Tris yog siv rau cov protein siv lead ua kev loj hlob nyob rau hauv cov pH sib txawv.Lub zog qis ionic ntawm Tris tsis tuaj yeem siv rau kev tsim cov fibers nruab nrab ntawm lamin hauv C. elegans.Tris kuj yog ib qho tseem ceeb ntawm cov protein electrophoresis buffers.Tsis tas li ntawd, Tris yog qhov nruab nrab hauv kev npaj cov surfactants, vulcanization accelerators, thiab qee cov tshuaj.Tris kuj tseem siv los ua tus qauv titration.
Nyob rau hauv daim ntawv ntawm cov duab thiab cov ntawv nyeem, nws tuaj yeem suav nrog cov ntaub ntawv khoom, kev pabcuam thiab qhov zoo.Nws tuaj yeem mus dhau ntawm cov khoom ntxiv rau qee cov ntsiab lus (cov neeg siv khoom muaj kev txhawj xeeb, kev txhawj xeeb, thiab lwm yam), kev tiv thaiv ib puag ncig, kuaj dawb, thiab lwm yam.
1. Cov qauv raug kuaj dawb kom ntseeg tau tias kev siv cov neeg siv khoom ib txwm muaj.
2. Raws li qhov kev thov ntawm cov neeg siv khoom sib txawv, me me mus rau 100g, loj rau tons ntawm cov thoob, tuaj yeem ua tau raws li qhov yuav tsum tau ua ntawm ntim.
3. Cov nqe lus them nqi yooj yim, hloov xov tooj lossis kev lees txais (ua tau raws li kev txais tos)
4. Kev thauj mus los sai, tib hnub lossis hnub tom qab tuaj yeem xa tuaj, tag nrho cov txheej txheem ntawm kev taug qab cov ntaub ntawv logistics, kom ntseeg tau tias cov neeg siv khoom siv sijhawm.
5. Kev pab cuam tom qab muag zoo heev, ua siab ntev thiab ua tib zoo daws txhua yam teeb meem uas yuav tshwm sim, xws li cov teeb meem zoo, koom tes nrog cov neeg siv khoom kom nquag plias, tsis txhob shirk lub luag hauj lwm thiab passive teb.
6. Pab neeg ua haujlwm zoo, kev ua haujlwm zoo thiab kev paub txog kev ua haujlwm ua rau cov neeg siv khoom tsis tsuas yog so kom paub meej txog peb cov khoom, tab sis kuj xav tias peb pab neeg txhim khu kev qha.
7. Kev ywj pheej xa tawm txoj cai los tsim kev lag luam thoob ntiaj teb thiab lub ntiaj teb hom kev cuam tshuam.
8. Ua siab ncaj, tsab ntawv raws li, 20 xyoo ntawm lub tuam txhab keeb kwm thiab lub koob npe nrov, cia cov neeg siv khoom yooj yim dua, kev sib nrig sib pab thiab yeej-yeej, kev koom tes ncaj ncees.
